Interdisziplinäres Zentrum für Materialwissenschaften
  Publikationen [suche]   
Organisation
Aktivitäten
Kontakt
Angebote für Studenten
Bereiche im
      Nanotechnikum
Martin-Luther-Universität
Interdisziplinäres Zentrum für Materialwissenschaften
Nanotechnikum Weinberg
Heinrich-Damerow-Str. 4,
D-06120 Halle, Germany
Tel.: +49 345 55 28471
Telefax:+49 345 55 27390 email: info@cmat.uni-halle.de
[Veröffentlichungen] [Graduierungsarbeiten] [Berichte] [Poster]
Abstracts

Pegah Esmaeilzadeh, Alexander Köwitsch, Frank Heyroth, Georg Schmidt, Steffen Fischer, Katja Richter, Thomas Groth
Synthesis of thiolated polysaccharides for formation of polyelectrolyte multilayers with improved cellular adhesion
CARBOHYDRATE POLYMERS 157 (2017), 1205-1214

Intrinsic cross-linking is not only useful for increasing stability, but also for tailoring mechanical properties of polyelectrolyte multilayers (PEM) on implants and tissue engineering scaffolds. Here, a novel route for synthesizing thiolated chitosan (t-Chi) based on the application of 3,3'-dithiodipropionic acid was applied, while thiolated chondroitin sulfate (t-CS) was conjugated by 3,3'-dithiobis (propanoic hydrazide). Both products were subsequently reduced to obtain the free thiols. The thiol content, structural changes and degree of substitution were studied by UV-vis, FTIR, Raman and H-1 NMR spectroscopy, respectively. Chi and CS can be used for PEM formation with the layer-by-layer method, due to the cationic nature of Chi at pH values below 5.0 and the anionic character of CS. Comparative studies on the formation of native Chi/CS versus t-Chi/t-CS PEM with surface plasmon resonance and ellipsometry revealed higher layer mass. We also found that the PEM composed of t-Chi/t-CS had superior cell adhesion properties for human keratinocytes in comparison to the native PEM.

Keywords: Polysaccharides; Thiolated chondroitin sulfate; Thiolated chitosan; Layer-by-layer technique; Cell adhesion; Bioactive coatings

DOI10.1016/j.carbpol.2016.10.088


Impressum Copyright © Center of Materials Science, Halle, Germany. All rights reserved.